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Título del libro: Globins And Other Nitric Oxide-Reactive Proteins, Pt A
Título del capítulo: A self-induction method to produce high quantities of recombinant functional flavo-leghemoglobin reductase

Autores UNAM:
JOSE RAUL ARREDONDO PETER;
Autores externos:
Idioma:
Inglés
Año de publicación:
2008
Resumen:

Ferric leghemoglobin reductase (FLbR) is able to reduce ferric leghemoglobin (Lb(3+)) to ferrous (Lb(2+)) form. This reaction makes Lb functional in performing its role since only reduced hemoglobins bind O(2) . FLbR contains FAD as prosthetic group to perform its activity. FLbR-1 and FLbR-2 were isolated from soybean root nodules and it has been postulated that they reduce Lb(3+). The existence of Lb(2+) is essential for the nitrogen fixation process that occurs in legume nodules; thus, the isolation of FLbR for the study of this enzyme in the nodule physiology is of interest. However, previous methods for the production of recombinant FLbR are inefficient as yields are too low. We describe the production of a recombinant FLbR-2 from Escherichia coli BL21(DE3) by using an overexpression method based on the self-induction of the recombinant E. coli. This expression system is four times more efficient than the previous overexpression method. The quality of recombinant FLbR-2 (based on s

Entidades citadas de la UNAM:
Fuente:



ISBN:
9780123742773
Editorial:
ELSEVIER ACADEMIC PRESS INC

Tipo de producto:
Review



DOI:
10.1016/S0076-6879(08)36023-6