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SISTEMA INTEGRAL DE INFORMACIÓN ACADÉMICA - PÚBLICO
Título del libro: Methods In Enzymology, Volume 484: Constitutive Activity In Receptors And Other Proteins, Part A Título del capítulo: alpha(1D)-ADRENERGIC RECEPTORS: CONSTITUTIVE ACTIVITY AND REDUCED EXPRESSION AT THE PLASMA MEMBRANE
Adrenergic receptors are a heterogeneous family of the G protein-coupled receptors that mediate the actions of adrenaline and noradrenaline. Adrenergic receptors comprise three subfamilies (alpha(1), alpha(2), and beta, with three members each) and the alpha(1D)-adrenergic receptor is one of the members of the alpha(1) subfamily with some interesting traits. The alpha(1D)-adrenergic receptor is difficult to express, seems predominantly located intracellularly, and exhibits constitutive activity. In this chapter, we will describe in detail the conditions and procedures used to determine changes in intracellular free calcium concentration which has been instrumental to define the constitutive activity of these receptors. Taking advantage of the fact that truncation of the first 79 amino acids of alpha(1D)-adrenergic receptors markedly increased their membrane expression, we were able to show that constitutive activity is present in receptors truncated at the amino and carboxyl termini, w
