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Título del libro: 2008 5th Ieee International Symposium On Biomedical Imaging: From Nano To Macro, Proceedings, Isbi
Título del capítulo: Three-dimensional image acquisition system for multi-sperm tracking

Autores UNAM:
GABRIEL ISAAC CORKIDI BLANCO; BLANCA ITZELT TABOADA RAMIREZ; CHRISTOPHER DAVID WOOD; ALBERTO DARSZON ISRAEL;
Autores externos:

Idioma:

Año de publicación:
2008
Palabras clave:

Motility; Sperm tracking; Three-dimensional analysis


Resumen:

Sperm motility has been widely studied in two dimensions (2D) by analyzing their bidimensional trajectories when swimming near a flat surface. Under real conditions, the spermatozoid swims in a three-dimensional space before finding its target, the egg. The main restriction to track three-dimensionally these flagellated cells is their speed. Here we describe a novel method that allows the acquisition of data for three dimensional multi-tracking and analysis of free-swimming sperm trajectories. The system uses a piezo-electric device displacing a large focal-distance objective mounted on an inverted microscope (over its optical axis) to acquire 70 stacks of 60 images at a rate of 4,200 images of 512 x 512 pixels per second, over a depth of 100 ?m. With this method whole 3D paths could be visualized during 1 s and measured for multiple sperm in the same field simultaneously for the first time. This new acquisition method extends the analytic possibilities from a restricted 2D situation to real 3D conditions. ©2008 IEEE.


Entidades citadas de la UNAM: