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Título del libro: 2010 Annual International Conference Of The Ieee Engineering In Medicine And Biology Society, Embc'10
Título del capítulo: Three dimensional template matching segmentation method for motile cells in 3D+t video sequences

Autores UNAM:
JAIME ARTURO PIMENTEL CABRERA; GABRIEL ISAAC CORKIDI BLANCO;
Autores externos:

Idioma:
Inglés
Año de publicación:
2010
Palabras clave:

3D video; Biological preparation; Biological samples; Equivalent size; Experimental data; Focal Plane; High rate; Motile cells; Sea urchin; Segmentation cell; Segmentation methods; Segmentation techniques; True positive; Video sequences; Extraction; Mammals; Optical properties; Template matching; Three dimensional; algorithm; animal; article; automated pattern recognition; cell culture; cell tracking; computer assisted diagnosis; cytology; image enhancement; image subtraction; male; methodology; reproducibility; sea urchin; sensitivity and specificity; spermatozoon; three dimensional imaging; video microscopy; Algorithms; Animals; Cell Tracking; Cells, Cultured; Image Enhancement; Image Interpretation, Computer-Assisted; Imaging, Three-Dimensional; Male; Microscopy, Video; Pattern Recognition, Automated; Reproducibility of Results; Sea Urchins; Sensitivity and Specificity; Spermatozoa; Subtraction Technique


Resumen:

In this work, we describe a segmentation cell method oriented to deal with experimental data obtained from 3D+t microscopical volumes. The proposed segmentation technique takes advantage of the pattern of appearances exhibited by the objects (cells) from different focal planes, as a result of the object translucent properties and its interaction with light. This information allows us to discriminate between cells and artifacts (dust an other) with equivalent size and shape that are present in the biological preparation. Using a simple correlation criteria, the method matches a 3D video template (extracted from a sample of cells) with the motile cells contained into the biological sample, obtaining a high rate of true positives while discarding artifacts. In this work, our analysis is focused on sea urchin spermatozoa cells but is applicable to many other microscopical structures having the same optical properties. © 2010 IEEE.


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