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Título del libro: Proceedings Of The 19th International Symposium On Medical Information Processing And Analysis, Sipaim 2023
Título del capítulo: Improving FLIM Resolution with Mean-Shift Super-Resolution Microscopy, an analytical approach

Autores UNAM:
ADAN OSWALDO GUERRERO CARDENAS;
Autores externos:

Idioma:

Año de publicación:
2023
Palabras clave:

Diffraction; Fluorescence imaging; Image resolution; Medical imaging; Optical transfer function; Analytical approach; Deconvolutions; Fluorescence lifetime imaging microscopy; Mean shift; Mean-shift super-resolution microscopy; Molecular properties; Point-Spread function; Spatial resolution; Super-resolution microscopy; Superresolution; Fluorophores


Resumen:

Fluorescence Lifetime Imaging Microscopy (FLIM) is utilized to study the spatial distribution of fluorophores and assess molecular properties within cells or tissues. However, FLIM faces spatial and lifetime blurring due to the convolution of fluorophore light with the microscope's point spread function, diminishing spatial resolution and affecting lifetime distributions. In this research, we developed a model based on photophysical principles to examine the impact of the Mean-Shift Super-Resolution microscopy (MSSR) approach on FLIM's spatial and lifetime resolution. Through simulations of isolated fluorophores with lifetimes of 1.0 and 3.0 ns, we demonstrated that MSSR enhances spatial resolution beyond the diffraction limit and reduces lifetime blurring. Experimental validations will be beneficial to further support these findings, potentially contributing to increased accuracy in various medical and biomedical imaging applications. © 2023 IEEE.


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