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Título del libro: Biomolecules In Organic Solvents
Título del capítulo: ENZYMES IN REVERSE MICELLES CONTAINING PHOSPHOLIPIDS

Autores UNAM:
ALBERTO DARSZON ISRAEL;
Autores externos:

Idioma:

Año de publicación:
2024
Resumen:

A considerable effort is now being devoted to the study of enzymes in media that are predominantly composed of organic solvents. 1?13 There are multiple reasons for this, among which some of the main ones are Many interesting and industrially important reactions must take place in hydrophobic environments with apolar substrates. Thus, resuspending or trapping functional enzymes, which are extremely competent catalysts, in hydrophobic environments has become very attractive. In addition, these systems also allow the use of soluble substrates. In such apolar environments, under certain conditions, enzymes behave quantitatively and/or qualitatively different than in aqueous media. Some gain enormously in thermal stability, and others are capable of performing reactions that do not occur to a significant degree in water, i.e., reversing hydrolytic reactions. Water participates directly or indirectly in all processes leading to protein thermal denaturation. 4?6 Thus, it is clear that systems which allow the experimenter to control the amount of water to which the enzyme is exposed can be advantageously used to study the water requirements of enzymatic catalysis and the participation of this solvent in thermostability and protein structure. © 1992 by Taylor & Francis Group, LLC.


Entidades citadas de la UNAM: